He demonstrated how NMR data could be used to identify protonation states of proteins and to follow coupled equilibria and define thermodynamic properties of biological macromolecules, including multiple conformational states. As an assistant professor, his studies were enabled by access to NIH-funded NMR instrumentation at Carnegie Mellon University. To pay this forward for the next generations of young investigators, Professor Markley founded the Purdue University Biological Magnetic Resonance Laboratory and subsequently the National Magnetic Resonance Facility at Madison. His laboratory carried out early studies of multinuclear NMR spectroscopy, in particular the use of low level 13C labeling and 13C detection of 1H-13C and 13C-13C interactions. He developed efficient recombinant DNA technology for producing stable isotope labeled proteins from cells and cell-free extracts. His group developed methods for determining the strengths of hydrogen bonds and the influence on hydrogen bonding on the redox potentials of metalloproteins. He helped elucidate enzyme mechanisms and the pathway of iron-sulfur cluster assembly. Professor Markley and his associates stressed the importance of archiving validated NMR parameters and founded the Biological Magnetic Resonance Data Bank (BMRB) and guided its development and affiliation with the Protein Data Bank. The Markley group developed and disseminated software tools for NMR parameter identification, assignment, and structure determination. The group carried out early studies of NMR-based metabolomics and developed freely accessible libraries of NMR parameters of metabolites.
We call for reviews, forward looking perspectives, and original articles influenced by or related to Professor Markley's work.
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